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Measurements of respiratory activity in isolated protoplasts of Aspergillus nidulans

✍ Scribed by A.V. Gokhale; Susan Isaac

Book ID
117070780
Publisher
Elsevier
Year
1984
Weight
298 KB
Volume
83
Category
Article
ISSN
0007-1536

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Transformation of filamentous fungi with DNA has been hampered by the low frequencies generally obtained. A variety of methods have been explored, most relying on the enzymatic removal of the cell wall (protoplasting) before DNA treatment (reviewed in (1)). Dithiothreitol (DTT) 4 has been shown to i