Several methods are available for the extraction and quantitation of oxidized and reduced pyridine nucleotides in erythrocytes. Enzymatic methods, however, are complicated by the presence of hemoglobin, which causes oxidation of NADH and NADPH during extraction. Although hemoglobin-mediated oxidatio
โฆ LIBER โฆ
Measurements of reduced pyridine nucleotides in a single neuron
โ Scribed by C.A. Terzuolo; B. Chance; E. Handelman; L. Rossini; P. Schmelzer
- Book ID
- 113401084
- Publisher
- Elsevier Science
- Year
- 1966
- Weight
- 742 KB
- Volume
- 126
- Category
- Article
- ISSN
- 0926-6585
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A simplified and reliable assay for the determination of erythrocyte pyridine nucleotide (NAD and NADP) concentrations, as well as the ratio of the reduced [NADH/NADPH] to oxidized \(\left[\mathbf{N A D}^{+} / \mathbf{N A D P}^{+}\right.\)] nucleotide, is important in understanding both normal and a