𝔖 Bobbio Scriptorium
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Measurements of pertinent concentrations of oxygen in vivo

✍ Scribed by H. M. Swartz; S. Boyer; P. Gast; J. F. Glockner; H. Hu; K. J. Liu; M. Moussavi; S. W. Norby; N. Vahidi; T. Walczak; M. Wu; R. B. Clarkson

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
331 KB
Volume
20
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

A new method able to measure the concentration of oxygen in complex biological systems, including in vivo, has been developed using low‐frequency EPR and newly characterized free radicals that are very sensitive to the concentration of oxygen. The free radicals (fusinite and lithium phthalocyanine) are very stable in tissues (for at least 150 days), apparently nontoxic, and can reflect oxygen concentrations that are less than the K~m~ of cytochrome oxidase (0.1 ΞΌ__M__ or lower). Their biological stability is indicated by the fact that repeated measurements with fusinite of the concentration of oxygen in skeletal muscle have been made in the same animal for more than 150 days without any change in sensitivity or signs of toxicity. Β© 1991 Academic Press, Inc.

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