Measurement of15N relaxation rates in perdeuterated proteins by TROSY-based methods
β Scribed by Nils-Alexander Lakomek, Jinfa Ying, Ad Bax
- Book ID
- 118801763
- Publisher
- Springer Netherlands
- Year
- 2012
- Tongue
- English
- Weight
- 682 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0925-2738
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π SIMILAR VOLUMES
Dynamic parameters become more and more accessible in the study of uniformly isotopically enriched proteins by MAS solid-state NMR. We demonstrate that T 2 -related relaxation properties can quantitatively be determined in a sample of a perdeuterated microcrystalline protein by the measurement of 15
Internal motions play an important role in the biological function of proteins and NMR relaxation studies may characterize them over a wide range of frequencies. An experimental pulse scheme is proposed for the measurement of the 13C0-13C ~ cross-relaxation rate. For sensitivity reasons, this measur