Measurement of UV radiation using suspensions of microorganisms
β Scribed by Regina Sommer; Alexander Cabaj; Tudor Sandu; Miranda Lhotsky
- Book ID
- 104373536
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 212 KB
- Volume
- 53
- Category
- Article
- ISSN
- 1011-1344
No coin nor oath required. For personal study only.
β¦ Synopsis
The measurement of solar UV radiation is usually performed using physical devices like photodiodes or photomultipliers or with chemical substances (actinometry). The application of biological material such as microorganisms for this purpose has gained increasing importance in the last few years. The microorganisms may be dried and spread on a flat surface or they may be in aqueous suspensions contained in UVtransparent vessels. If the measurements are done on flat surfaces, the irradiance weighted by the action spectrum of the dried microorganism used is the result of the measurement. If aqueous suspensions of microorganisms are used, contained for instance in spherical vessels, the fluence weighted by the action spectrum of the microorganisms in the aqueous suspension is the result. A problem of this method of measurement can be that inside the vessel the distribution of UV radiation is usually not homogeneous, causing distributions of fluences among the irradiated microorganisms, which may result in variation of the results depending on the mixing characteristics of the suspension during irradiation.
π SIMILAR VOLUMES
The depletion of stratospheric ozone and the health risks associated with the consequent expected increase in UVB radiation have prompted efforts to measure solar UV radiation using a variety of different techniques.
## Abstract Multiwavelength transmission spectra of microorganisms and cell suspensions consist of combined absorption and scattering phenomena resulting from the interaction of light with microorganisms or cells typically suspended in a nonabsorbing media. The distribution of intensities as a func