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Measurement of urinary somatomedin C by a chemiluminescent method

✍ Scribed by Orlando, Claudio ;Tode, Bettina ;Pinzani, Pamela ;Bassi, Francesca ;Messeri, Gianni

Publisher
John Wiley and Sons
Year
1989
Weight
317 KB
Volume
3
Category
Article
ISSN
0884-3996

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✦ Synopsis

We present a method for the measurement of the total Somatomedin C (SmC) content in human early morning urine samples after dialysis, extraction, and concentration. We modified a chemiluminescence immunoassay, previously developed for' SmC determination in serum, for analysis of SmC in urine. Appropriate sensitivity was obtained by the preparation of a new chemiluminescent tracer (AEECCOOHSmC) and the optimization of a competitive non-equilibrium immunoassay system which had a detection limit of 0.24 fmol SmC per tube.

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