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Measurement of uric acid, ascorbic acid, and related metabolites in biological fluids

✍ Scribed by Linda L. Hatch; Alex Sevanian

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
419 KB
Volume
138
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid and quantitative method for the simultaneous measurement of uric acid, ascorbic acid, and related metabolites in biological fluids is described. This method makes use of anionexchange extraction columns to isolate the desired compounds from a wide range of biological fluids, and anionexchange HPLC with ultraviolet detection for their separation and measurement. By this procedure the contents of uric and ascorbic acids in human serum and urine were estimated. Furthermore, the oxidation of uric acid to allantoin and ascorbic acid to dehydroascorbic acid could be readily monitored.

KEY WORDS high-pressure liquid chromatography; anionexchange extraction chromatography.

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