Measurement of translational displacement probabilities by NMR: An indicator of compartmentation
β Scribed by D. G. Cory
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 512 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0740-3194
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β¦ Synopsis
Abstract
We introduce and demonstrate an NMR pulsed gradient stimulated echo method of directly obtaining the molecular translational displacement probability (displacement profile) of a liquid. The temporal development of the displacement profile reflects the presence of diffusion, restrictions to diffusion (e.g., walls, membranes), flow, and spatially dependent relaxation sinks. This approach allows the study of compartments which are too small to be observed by conventional NMR imaging methods. The distribution of spatial properties of compartments can be characterized over a spatial field of about 0.1 to 25 ΞΌm, completely independent of the absolute spatial location of the individual compartments. Β© 1990 Academic Press, Inc.
π SIMILAR VOLUMES
An experimental strategy for determining the extent to which multiply isotopically labeled fragments are incorporated intact into relatively complicated compounds of interest is presented. The NMR methods employed are based on isotope-filtered one-dimensional spectra and difference HSQC spectra inco