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Measurement of thyroid hormone in the rat thyroid gland by radioimmunoassay

✍ Scribed by Philippe Chomard; Nicole Autissier

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 504 KB
Volume: 192
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90539-6

No coin nor oath required. For personal study only.

✦ Synopsis

The present paper describes (i) a hydrolysis technique with Pronase and leucine aminopeptidase using one rat thyroid gland, resulting in maximum release of thyroid hormones and minimum deiodination, and (ii) a simple and rapid procedure for thyroid hormone radioimmunoassays in thyroid hydrolysates using commercial kits intended for serum thyroid hormone determinations. The procedure is used to determine T4, T3, and rT3 concentrations and hormonal molar ratios in a thyroid gland from a male Wistar rat. The reliability of the method is also studied.

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