Measurement of the malondialdehyde–2′-deoxyguanosine adduct in human urine by immuno-extraction and liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry

✍ Scribed by Anne-Mette Hoberg; Michael Otteneder; Lawrence J. Marnett; Henrik E. Poulsen

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 124 KB
• Volume: 39
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.547

✦ Synopsis

Abstract

The major adduct of malondialdehyde with guanine, M~1~G, was measured in human urine from non‐smoking healthy individuals. M~1~G is a mutagenic DNA lesion and a terminal product of lipid peroxidation in vivo that may be implicated in cancer related to lifestyle and diet. On the basis of a recently developed method for the quantification of M~1~G as an excreted deoxynucleoside using immuno‐extraction purification, chemical NaBH~4~ reduction and liquid chromatography combined with atmospheric pressure chemical ionization tandem mass spectrometry, we demonstrate that the average 24 h excretion rate of M~1~G‐dR is about 12 ± 3.8 fmol kg^−1^ (n = 5). Copyright © 2004 John Wiley & Sons, Ltd.