Measurement of testosterone in infant fecal samples

✍ Scribed by Amanda L. Thompson; Patricia L. Whitten; Michelle Lampl

• Book ID: 101442516
• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 123 KB
• Volume: 23
• Category: Article
• ISSN: 1042-0533
• DOI: 10.1002/ajhb.21205

✦ Synopsis

Abstract

Objectives:

This study reports the validation of a noninvasive method for repeated assessment of testosterone from infant fecal samples.

Methods:

Fecal samples were collected from cotton diaper liners, subjected to methanol extraction, and assayed using a modified commercial testosterone RIA kit.

Results:

Method validity was supported by a recovery near 100%, a sensitivity of 1.23 pg/ml, and inter‐ and intra‐assay coefficients of variations less than 10 and 15%, respectively. Testosterone was detected in all samples from male and female infants aged 2 weeks to 15 months.

Conclusions:

Fecal assessment is supported as a novel, non‐invasive tool for studying testosterone during early human development. Am. J. Hum. Biol., 2011. © 2011 Wiley‐Liss, Inc.