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Measurement of steroid hormones in plasma by isocratic high performance liquid chromatography coupled to radioimmunoassay

✍ Scribed by Stefano Boschi; Rosaria De Iasio; Paolo Mesini; Gian Franco Bolelli; Raffaella Sciajno; Renato Pasquali; Maurizio Capelli


Book ID
115828125
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
508 KB
Volume
231
Category
Article
ISSN
0009-8981

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Branched-chain ketoacids were isolated from plasma or serum samples by acidification, passage through a cationic exchange resin, ether extraction, and extraction of the ether layer with phosphate buffer. The recovery of 2-[ l-14C]ketoisocaproate taken through these procedures averaged 95 + 3%. Branc