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Measurement of Specific Protease Activity Utilizing Fluorescence Polarization

✍ Scribed by Leanna M. Levine; Marshall L. Michener; Mihaly V. Toth; Barry C. Holwerda


Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
117 KB
Volume
247
Category
Article
ISSN
0003-2697

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## Background: The Cellscan mark-S (CS-S) scanning cytometer was used for tracing enzymatic reactions in the same individual cells under various physiological conditions over periods of minutes. On-line reagent addition and changes in the experimental conditions (buffers, ions, substrates and inhib