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Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy

✍ Scribed by Jesse D. McCool; Edward Long; Joseph F. Petrosino; Hilary A. Sandler; Susan M. Rosenberg; Steven J. Sandler


Book ID
110832145
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
466 KB
Volume
53
Category
Article
ISSN
0950-382X

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We have examined the level of expression of the SOS regulon in cells lacking DNA adenine methylase activity (darn-). Mud (Ap, lac) fusions to several SOS operons (recA, lexA, uvrA, uvrB, uvrD, sulA, dinD and dinF) were found to express higher levels of fl-galactosidase in darn-strains than in isogen