Measurement of sodium concentration in physiological solutions by means of an electrode made from glass B 104
✍ Scribed by D.M. Nutbourne
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 577 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
For some years it has been possible to measure the H+ concentration of solutions by means of electrodes made from a glass sensitive to H+. Now it is possible to obtain similar electrodes made from glass sensitive to Na+. Eisenman and his colleagues (13), Friedman and his associates (4-7)) Moore and Wilson (8)) Portnoy and Gurdjian (9), and Sugioka (10) have all described how such electrodes can be satisfactorily used to determine the sodium in body fluids. Their electrodes were made from the series of glasses NAS 11-18. The experiments described below were performed using electrodes made from a different glass, BH 104, which was devised and investigated by . The purpose of the present investigation was to evaluate the use of these electrodes in measuring the sodium in fluids whose chemical composition resembles that of blood. In most of the experiments described the estimations were carried out on undiluted 2-3 ml samples of Ringer solutions that had been in contact with living membranes. A few experiments were also carried out on blood. METHOD Apparatus This consisted essentially (Fig. 1) of a Natsensitive electrode, M, made from glass B 104, and a calomel half-cell reference electrode, A; and the potential difference between them was a function of the Na+ activity in the sample, L. The glass electrode, supplied by Electronic Instruments Ltd., was connected to the high-impedance