Measurement of serum Lp(a) by COBAS MIRA using a latex immunoturbidimetric assay kit

✍ Scribed by Seiichi Fujita; Takahiro Sano; Yoshiaki Katayama

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 308 KB
• Volume: 8
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860080608

✦ Synopsis

We had an opportunity to test a reagent kit for serum lipoprotein (a) [Lp(a)] assay, which was based on the latex immunoturbidimetric assay method, and applied it to COBAS MIRA (Roche). Reproducibility of the assay procedure was 0 . 9 2 4 0 % CV of three samples which contained 12.58-60.4 mgldl of Lp(a). Minimum detectable concentration was 1.5-2.0 mg/dl. It was confirmed that in-terference of co-existing substances, i.e. hemoglobin, bilirubin, and intralipid, was negligible. No cross reactivity was seen with plasminogen. Correlation with a ELISA method was excellent. Frequency distribution of Lp(a) in healthy Japanese was similar to that found in white population.