The expression of regucalcin, a regulatory protein of Ca(2+) signaling, and its effect on Ca(2+) pump activity in the microsomes (sarcoplasmic reticulum) of rat heart muscle was investigated. The expression of regucalcin mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PC
Measurement of Sarcoplasmic Reticulum Ca2+-ATPase Activity and E-Type Mg2+-ATPase Activity in Rat Heart Homogenates
✍ Scribed by Ana Saborido; Jerónimo Delgado; Alicia Megı́as
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 108 KB
- Volume
- 268
- Category
- Article
- ISSN
- 0003-2697
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## Abstract The role of regucalcin, which is a regulatory protein in intracellular signaling, in the regulation of Ca^2+^‐ATPase activity in the mitochondria of brain tissues was investigated. The addition of regucalcin (10^−10^ to 10^−8^ M), which is a physiologic concentration in rat brain tissue