Measurement of prostaglandin biosynthetic capacity in discrete areas of the rabbit hippocampal formation

✍ Scribed by T. W. Lysz; P. Needleman; J. A. Ferrendelli

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 518 KB
• Volume: 10
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490100310

✦ Synopsis

Assay conditions sensitive enough to measure prostaglandin (PG) hiosynthctic capacity in discrete brain areas have been dcvcloped. Serial sections of the rabbit hippocampal formation were dissected from the brain, frozen, and 16 pm scctions w u c cut and freeze dried. The assay was performed with 40-200 p g dry weight of tissue in a 30 pl incubation volume containing high specific activity arachidonic acid. The whole hippocampal formation slice or its subdivisions (subiculuni. dentate gyrus, and hippocarnpus) when assayed individually demonstraled the ability to produce approximately 30-50 pmolimg dry weight PGF2,*. PGE2, and lesser amounts of PGDl (in a ratio of 1:0.8:0.3). A modest yet significant increase in PGE? production was nieasured in the dentate gyrus when compared to PGE? formation in the subiculum or hippocampus. An intact PGE? isomerase was confirmed in the hippocarnpal formation slice by incubations with PGH? endoperoxide. These results demonstrate that quantitative histochemical techniques can be used to measure PG metabolism in microscopic regions of brain.