Measurement of precursors for α-amidated hormones by radioimmunoassay of glycine-extended peptides after trypsin-carboxypeptidase B cleavage
✍ Scribed by Linda Hilsted; Jens F. Rehfeld
- Book ID
- 102984975
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 560 KB
- Volume
- 152
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Using fragment 5-I7 of human gastrin-I7 extended with glycine at the C-terminus as hapten, three of six rabbits produced high-titer, high-avidity antisera specific for glycine-extended gastrins. In combination with trypsin and carboxypeptidase B cleavage, radioimmunoassays based on these antisera measured progastrins in some extra-antral tissues and certain malignant tumors. The results show that sequential cleavage with trypsin and carboxypeptidase B followed by radioimmunoassay of glycine-extended peptides is a rapid and accurate procedure for measurement of biosynthetic precursors of ol-amidated peptide hormones. Moreover, the procedure seems promising in the search for tumor markers. 0 1986 Academic press. Inc.