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Measurement of N-methyl-2-pyridone-5-carboxamide in urine by high performance liquid chromatography

✍ Scribed by William P. T. Moore; Colin H. Bolton; Leonie Downs; Hilary A. Gilmor; Edwin A. M. Gale

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 44 KB
Volume: 14
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(200004)14:2<69::aid-bmc922>3.0.co;2-n

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✦ Synopsis

We have previously described a simple and reproducible method for the measurement of nicotinamide and its major metabolite N-methyl-2-pyridone-5-carboxamide (2-pyr) in human plasma. We now describe a low-cost high-throughput method for measurement of urinary 2-pyr, and demonstrate that Isolute C18 bulk can replace use of the column to clean up the samples prior to injection into the HPLC apparatus. Using a standard curve together with an internal standard for each sample, with mean recovery of 2-pyr greater than 95%, the assay has proved reproducible, with considerable savings in cost and time. The principal advantages of this method are the rapid column clean up of samples prior to injection and the simple but effective methodology

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