Measurement of myo-inositol in single cells and defined areas of the nervous system by selected ion monitoring

✍ Scribed by William R. Sherman; Paul M. Packman; Michael H. Laird; Rita L. Boshans

• Book ID: 102982608
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 811 KB
• Volume: 78
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90015-x

✦ Synopsis

In this work we want to bring attention to the potential value of the direct analysis of tissue constituents using two well established techniques: the selected ion monitoring method of gas chromatography-mass spectrometry and the techniques of quantitative histochemistry. The substrate analyzed is myo-inositol which is measured in single cells of rabbit Deiters' nucleus, spinal cord anterior horn, and dorsal root ganglion. Comparison of inositol levels in neurons vs neuropil showed a significantly greater level in anterior horn neurons; however, no difference was found in Deiters' nucleus. In dorsal root ganglion cells, 5-pm sections through a portion of the cell not containing the nucleus were compared with sections through a portion of the nucleus; no difference in inositol levels was found. Two hypothalamic nuclei were examined and found to have very different concentrations of inositol. In the samples examined, there was a threefold inositol concentration range.