Measurement of limiting mobilities by capillary isotachophoresis with a constant temperature at the site of detection

✍ Scribed by Bohuslav Gaš; Jiří Zuska; Jiří Vacík

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 567 KB
• Volume: 470
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94200-6

✦ Synopsis

A method is described for the determination of limiting mobilities from isotachophoretic experiments. The data from an universal detector in the isotachophoretic apparatus were fed to an on-line computer and recalculated to the actual resistance. The computer simultaneously regulates (on the basis of the calculated actual resistance in the detected zone) the driving current passing through the capillary tube, so that the thermal power at the detection site is constant and the temperature of the test solution has a required (preferably standard) value. An advantage of an isotachophoretic experiment controlled in this way is the fact that the dependence of the physical and physico-chemical quantities used on the temperature need not be considered in the recalculation of the experimental data to the limiting ionic mobilities. The limiting mobility calculation considers all the substances as ampholytes. The method was verified using an apparatus with an high-frequency contactless detector.