Measurement of ketone bodies in subcellular fractions using a spectrophotometric iron-chelate assay

✍ Scribed by R.I. Kientsch-Engel; E.A. Siess; O.H. Wieland

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 464 KB
• Volume: 123
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90445-6

✦ Synopsis

A sensitive spcctrophotometric assay for 3-hydroxybutyrate determination in biological samples is described. Linearity between the amount of 3-hydroxybutyrate and A&& was obtained in the range of 0.3 to 4.0 nmol 3-hydroxybutyrate/assay. The same method is applicable for acetoacetate determination after its enzymatic reduction. The assay proved to be useful for the study of the subcellular distribution of ketone bodies in isolated liver cells. The assay procedure is adequate to measure the concentration of ketone bodies in 5-mg and 20-~1 samples from liver and blood, respectively.