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Measurement of intracellular cytokines

โœ Scribed by Uwe Schauer; Thomas Jung; Norbert Krug; Anthony Frew


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
410 KB
Volume
17
Category
Article
ISSN
0167-5699

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## Background: Identification of human t-helper cell subsets is possible by measurement of intracellular cytokines after coincubation of lymphocytes with phorbol myristate acetate (pma), calcium ionophore, and brefeldin a for up to 20 h. however, exposure to pma leads to internalization of membrane