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Measurement of Intracellular Calcium in Cell Populations Loaded with Aequorin: Neurokinin-1 Responses in U373MG Cells

✍ Scribed by Lynne Hedley; Stephen B. Phagoo; Iain F. James


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
106 KB
Volume
236
Category
Article
ISSN
0003-2697

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Variations in intracellular free calcium concentration (A1Cazfli) were measured in intact and isolated human astrocytoma cells (U373 MG) loaded with fura-2 acetoxymethylester. Microperfusion of 50 nM substance P (SP), applied for 1 s, increased [Ca" ' Ii by 351 nM from a stable basal level of [Ca2+l