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Measurement of ‘in situ’ mitochondrial membrane potential in Ehrlich ascites tumor cells during aerobic glycolysis

✍ Scribed by Jesús Sánchez Olavarría; Clara Galindo; Mayte Montero; Yolanda Baquero; Javier Vitorica; Jorgina Satrústegui


Book ID
115719447
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
683 KB
Volume
935
Category
Article
ISSN
0005-2728

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Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried i n continuous spinner culture have been synchronized. Simultaneous monitoring of :tH-thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hours. This is composed o