𝔖 Bobbio Scriptorium
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Measurement of GTPγS binding to specific G proteins in membranes using G-protein antibodies

✍ Scribed by Takashi Okamoto; Tsuneya Ikezu; Yoshitake Murayama; Etsuro Ogata; Ikuo Nishimoto


Book ID
115926555
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
362 KB
Volume
305
Category
Article
ISSN
0014-5793

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Three BODIPY GTPgammaS analogs (FL, 515, and TR), BODIPY FL GppNHp and BODIPY FL GTP molecules were synthesized as possible fluorescent probes to study guanine nucleotide binding spectroscopically. Binding to G(alphao) increases baseline analog fluorescence by 6-, 8.5-, 2.8-, 3.5-, and 3.0-fold, res