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Measurement of free and esterified carnitine in tissue extracts by high-performance liquid chromatography

โœ Scribed by Naoshi Takeyama; Daisuke Takagi; Kazuya Adachi; Takaya Tanaka

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
630 KB
Volume
158
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A high-performance liquid chromatographic (HPLC) method for the determination of L-camitine in clamped and frozen rat livers is described.

L-carnitine + acetyl-CoA P acetyk-camitine + CoASH

Using the above enzymatic reaction, release of CoASH is stoichiometric with the L-camitine added. The present method has made possible the determination of camitine in liver tissues, which is difficult by the conventional enzymatic spectrophotometric method using 5,5'-dithiobis(2nitrobenzoic acid), owing to acetyl-CoA hydrolysis during prolonged incubations at pH 7.8.

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