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Measurement of formaldehyde as dimedone adduct and potential formaldehyde precursors in hard tissues of human teeth by overpressured layer chromatography

✍ Scribed by T. K. Różyło; R. Siembida; E. Tyihák


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
103 KB
Volume
13
Category
Article
ISSN
0269-3879

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✦ Synopsis


Simple, automatic overpressured layer chromatographic methods for the parallel determination of endogenous formaldehyde in the form of dimedone adduct and potential formaldehyde generators (betaines) in hard tissues of human teeth are described together with an efficient densitometric evaluation. These simple procedures involve the special preparation of teeth and extraction of formaldehyde, of different binding force in teeth, with methanol containing dimedone, and the isolation of betaines with an aqueous solution of methanol.