Measurement of erythrocyte deformability and methodological adaptation for small-animal microsurgical models
✍ Scribed by Norbert Nemeth; Adrienn Gulyas; Anita Balint; Katalin Peto; Endre Brath; Ferenc Kiss; Istvan Furka; Oguz K. Baskurt; Iren Miko
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 137 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0738-1085
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✦ Synopsis
Abstract
Measuring erythrocyte deformability with bulk filtrometers is common, but there are problems with standardization and sample quantity in small laboratory animals. In this study, blood was drawn from mice, rats, and dogs, and then erythrocyte suspensions at 1–5% hematocrit were prepared. Bulk filtrometers with a 5‐ or 3‐μm pore‐diameter filter were used to determine the initial relative filtration rate (IRFR) and relative cell transit time (RCTT). Hematological composition was also tested. In all species, the highest IRFR values occurred at 1% hematocrit, and the lowest values at 5%. In mice and rats, RCTT values were lowest at 1% and highest at 5% using a 5‐μm filter, whereas in dogs, RCTT was elevated at 1–2%. Using a 3‐μm filter, each species showed the same phenomena under 3%. RCTT values may become distorted above a certain cell‐size/pore‐size ratio. In mice and rats, 1% suspension is applicable for bulk filtrometry, but only using 5‐μm filters. © 2006 Wiley‐Liss, Inc. Microsurgery 26: 33–37, 2006.