Rat colon mucosa microsomes contain a competent mixed function oxidase system that hydroxylates the N-methyl drugs benzphetamine and ethylmorphine, the 0-alkyl drugs p-nitroanisole and p-nitrophenetole and the polycyclic carcinogen benzo[a]pyrene. The colon system's hydroxylation activities can be s
Measurement of cytochrome P-450 in the presence of large amounts of contaminating hemoglobin and methemoglobin
β Scribed by Karin A.M. Johannesen; Joseph W. DePierre
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 457 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In this paper we apply luminol (5-amino-2,3-dihydro-1,4-phthalazinedione), a light-emitting substrate, in conjunction with \(\mathrm{H}_{2} \mathrm{O}_{2}\) to the luminescence labeling of hemoproteins. We describe in detail a photodetection device which permits an efficient recording of the light e
The concept of relative activity factor (RAF) to extrapolate data obtained with recombinant cytochrome P450(CYP)s to human liver microsomes has been proposed. To evaluate the approach to predict the contribution of multiple CYPs using RAF, we investigated the effects of the differences in the expres