Measurement of cell impedance in frequency domain using discontinuous current clamp and white-noise-modulated current injection
✍ Scribed by M. Weckström; E. Kouvalainen; M. Juusola
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 388 KB
- Volume
- 421
- Category
- Article
- ISSN
- 0031-6768
No coin nor oath required. For personal study only.
✦ Synopsis
A method is decribed for the determination of cellular input impedance of non-spiking neurones. The input impedance is important when cellular geometry and the effects of voltage-dependent channels are considered. Cells are impaled with a single glass microelectrod and current is injected using a time-sharing technique. The cell's impedance is measured by randomly modulating the injected current and calculating the impedance as a transfer function between current and recorded membrane voltage. Corresponding coherence functions can also be calculated for estimating the signal-to-noise ratio, and also linearity (i.e. possible activation of voltage-dependent conductances) of the membrane.