Measurement of Carbon–PhosphorusJCoupling Constants in RNA Using Spin–Echo Difference Constant-Time HCCH–COSY
✍ Scribed by Charles G. Hoogstraten; Arthur Pardi
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 122 KB
- Volume
- 133
- Category
- Article
- ISSN
- 1090-7807
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✦ Synopsis
We report a novel NMR technique for the measurement of carbon-phosphorus coupling constants in RNA oligomers. This method, spin-echo difference constant-time HCCH-COSY, takes advantage of the well-dispersed H1 and C1 resonances to analyze couplings involving the more poorly dispersed ribose carbon and phosphorus resonances. The technique was applied to analysis of the 3 J C2P coupling constants related to backbone ⑀ torsion angles in a 30-nucleotide lead-dependent ribozyme. 3 J C2P coupling constants were obtained for ϳ90% of the residues in this RNA, which is over twice as many as could be obtained with previous methods.