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Measurement of Binding of Adenine Nucleotides and Phosphate to Cytosolic Proteins in Permeabilized Rat-Liver Cells

โœ Scribed by Henk S. GANKEMA; Albert K. GROEN; Ronald J. A. WANDERS; Joseph M. TAGER

Book ID
115121685
Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
423 KB
Volume
131
Category
Article
ISSN
1432-1327

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โœ M.E. Peter; J. She; L.A. Huber; C. Terhorst ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 1019 KB

A novel approach for identification of adenine and guanine nucleotide-binding proteins in permeabilized cells is described. Cells were incubated for various periods with \(\alpha-{ }^{32} \mathrm{P}\)-labeled nucleotides and then briefly treated with periodate. Condensation products formed in situ b