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Measurement of anti-double-stranded DNA antibodies in major immunoglobulin classes

✍ Scribed by Shinichi Aotsuka; Masako Okawa; Kenji Ikebe; Ryuichi Yokohari


Book ID
118897848
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
720 KB
Volume
28
Category
Article
ISSN
0022-1759

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A simple and sensitive enzyme-linked immunosorbent assay (ELISA) is described for the measurement of antibodies to native DNA (double-stranded) and to heat-denatured DNA (single-stranded) in sera of patients with connective tissue disorders. DNA bound to polyvinylchloride plates is incubated with se