Measurement of amniotic fluid interleukin-6 using commercial kits
✍ Scribed by Tsunenobu Tamura; William W. Andrews; Kelley E. Johnston; G. Philamon Hemstreet; Robert L. Goldenberg
- Publisher
- Hindawi Publishing Corporation
- Year
- 1997
- Tongue
- English
- Weight
- 48 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1064-7449
No coin nor oath required. For personal study only.
✦ Synopsis
Objective: The association between increased amniotic fluid interleukin-6 (IL-6) concentrations and preterm labor has received increasing attention. Several research groups have evaluated this association using commercial IL-6 kits, which principally use the sandwich-enzyme-immunoassay method, and were originally created to measure IL-6 in plasma, serum, or culture media. We evaluated commercial kits for the determination of IL-6 in amniotic fluid.
Methods: Seven commercial kits were used to determine IL-6 concentrations in three amniotic fluid samples which were obtained from patients with clinical chorioamnionitis during labor and five from normal pregnancies at mid-trimester.
Results: Amniotic fluid IL-6 values differed significantly with some having over a 50-fold discrepancy and the recovery of known IL-6 added to amniotic fluid ranged from 12 to 123%. However, by all kits we were able to identify that amniotic fluid from patients with chorioamnionitis contained significantly higher IL-6 concentrations than those from normal mid-trimester pregnancies.
Conclusion: Our data indicate that standardization of the method for measuring IL-6 in amniotic fluid is desirable for the comparison of values from various laboratories. Infect. Dis. Obstet. Gynecol. 5:222-225, 1997.