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Measurement of acetylcholine receptor and anti-receptor antibodies by ELISA

✍ Scribed by Dr. N. L. Norcross; I. J. Griffith; J. A. Lettieri


Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
347 KB
Volume
3
Category
Article
ISSN
0148-639X

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✦ Synopsis


Abstract

Both acetylcholine receptor (AChR) and antibodies capable of binding to the AChR can be measured using the recently developed enzymelinked immunosorbent assay (ELISA). The assay is very sensitive and reproducible. Because of the relative simplicity of the ELISA in comparison to radioimmunoassay procedures currently used to measure anti‐AChR antibodies, the ELISA may prove valuable as a clinical diagnostic tool for the human disease myasthenia gravis.


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Myasthenia gravis (MG) is a neuromuscular disorder of man caused by a humoral response to the acetylcholine receptor (AChR). Most of the antibodies in MG and in experimental autoimmune myasthenia gravis (EAMG) are directed to the extracellular portion of the AChR alpha subunit, and within it, primar