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Measurement of 1J(Ni,Cαi), 1J(Ni,C′i−1), 2J(Ni,Cαi−1), 2J(HNi,C′i−1) and 2J(HNi,Cαi) values in 13C/15N-labeled proteins

✍ Scribed by Sulakshana Mukherjee; Sourajit M. Mustafi; H. S. Atreya; K. V. R. Chary

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
200 KB
Volume
43
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

Use of partial or selective ^13^C/^15^N labeling of specific amino acid residues in a given protein to measure the values of ^1^J(^15^N~i~,^13^C^α^~i~), ^2^J(^1^H^N^,^13^C^α^~i~), ^2^J(^15^N~i~,^13^C^α^~i−1~), ^1^J(^15^N~i~,^13^C′~i−1~) and ^2^J(^1^H^N^,^13^C′~i−1~) is described. This was achieved by recording a sensitivity‐enhanced 2D [^15^N–^1^H] HSQC experiment, without mixing the spin states of C^α^ and C′ during the course of entire experiment. Copyright © 2005 John Wiley & Sons, Ltd.

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