Measurement of 15N–1H bond lengths by rotational-echo double-resonance NMR spectroscopy

✍ Scribed by Riqiang Fu

• Book ID: 104108253
• Publisher: Elsevier Science
• Year: 2003
• Tongue: English
• Weight: 220 KB
• Volume: 376
• Category: Article
• ISSN: 0009-2614
• DOI: 10.1016/s0009-2614(03)00951-5

✦ Synopsis

A method based on rotational-echo double-resonance (REDOR) NMR is proposed to measure 1 H-15 N bond lengths under magic-angle-spinning. Protons are selectively polarized and evolve under the frequency-switched Lee-Goldburg (FSLG) homonuclear decoupling, while two 15 N p pulses are applied to restore 1 H-15 N dipolar interactions. Such a method relies on the 1 H-15 N dipolar couplings and requires no knowledge of their chemical shift tensors allowing one to precisely extract their couplings. This method could be applicable for measuring relatively long distances between amide protons and other nucleus such as 13 C if applying the p pulses to 13 C.