Measurement and Manipulation of Intracellular Ions, Volume 27, Methods in Neurosciences. Edited by Jacob Kraicer and S. Jeffrey Dixon

✍ Scribed by Evelyn F. Grollman

• Book ID: 102561511
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 24 KB
• Volume: 237
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1996.0221

✦ Synopsis

BOOK REVIEWS

Measurement and Manipulation of Intracellular Ions, Volume basis of the methods are covered, as is a description of NMR indicators of pH i , compartmentalization of pH, and the sources of errors 27, Methods in Neurosciences. Edited by JACOB KRAICER AND S. JEFFREY DIXON. Academic Press, San Diego, CA, 1995. 400 pp., with the method. The figures include typical results using the method; an inorganic phosphate spectrum from a preparation of rab-$95.00.

bit cerebrum is presented.

In addition to chapters devoted to one technique and one ion, there There are several reviews available describing techniques for meaare also general chapters on fluorescent indicators, ion-selective elecsuring cytosolic ions using (i) fluorescent indicators, (ii) ion-selective trodes, and NMR spectroscopy. Also discussed are the manipulation microelectrodes, and (iii) nuclear magnetic resonance (NMR) specof cytosolic pH and ionophores. All chapters are enhanced by serious troscopy. With this one volume, information on all three techniques discussion of disadvantages and problems encountered with each is provided in a manner that is concise, comprehensive, and sophistitechnique. In many instances, remedies for potential problems or cated. The book is a very practical guide for using these methods complications with methods are provided. Thus, not only advantages and has been prepared by experts. In addition to general chapters of a particular method are detailed, but also, just as importantly, on each method, there are individual chapters that provide complete practical aspects of their limitations are provided. coverage of a single method as applied to one ion. The discussion Examples of actual experiments using a specific technique are preincludes principles, practical guides for use, analysis of calibration sented for illustrative purposes. Although experimental material detechniques, and potential interference from other ions.

scribed is neural in origin, the editors are correct in assuming that Four chapters deal with calcium specifically and include fluoresmethods could easily be adapted to other systems. cence imaging and measurements as well as the use of chelators,

The book is highly recommended. The information is so comprephoto-activated caged compounds, and 19 F NMR indicators. The hensive and well presented that it is a necessary adjunct for both chapters on the use of fluorescent indicators, microelectrodes, and the novice and experienced investigator contemplating the measure-NMR to measure cytosolic sodium, magnesium, chloride, and pH are ment of intracellular ions. as thorough as those on calcium.

The depth of coverage of individual techniques is exemplified in EVELYN F. GROLLMAN NIDDK, NIH the chapter on cytosolic pH and NMR spectroscopy. The physical Methods in Enzymology, Volume 253, Adhesion of Microbial of TnphoA and T7 RNA polymerase to study fimbrial proteins, the molecular cloning of adhesion genes, and the molecular analysis of Pathogens. Edited by RON J. DOYLE AND ITZHAK OFEK. Academic Streptococcus pyogenes adhesin. The fifth section has nine articles Press, San Diego, 1995, 600 pp., $89.00.

on methods for the study of adhesion of selected bacteria. These include bacterial enteropathogens that must adhere to and penetrate This first volume of Methods in Enzymology devoted solely to adhethe intestinal mucosa with specific methods for various strains of sion of microbial pathogens complements two recent volumes of this enteropathogenic E. coli and of Salmonella typhimurium, uropathogenic strains of E. coli, Helicobacter pylori, the etiological agent of series (235 and 236) devoted to bacterial pathogenesis. It encomchronic type B gastritis, and Mollicutes (mycoplasmas), and three passes some fungal and protozoan pathogens in addition to prokaryarticles on the study of adhesion phenomena in oral bacteria. Section otes. The first section has six articles on general methods for studying six has four articles on the study of adhesion of eukaryotic pathogens microbial adhesion to animal cells including human phagocytes, including Trichomonas vaginalis, fungi, Entamaoeba histolytica, and mast cells, erythrocytes, ocular tissues, and the isolation of type 1

Candida albicans. The last section of eight articles returns to more fimbriated Escherichia coli from feces of orally challenged mice. The general methods with five articles covering methods for the study of second section contains six articles on the identification of adhesins adhesion to a diverse grouping of solid surfaces. These include plasand receptors including bacterial polysaccharide receptors that protic, extracellular matrix proteins, polymers and prosthetic devices, vide sites for adhesion of oral bacteria in dental plaque, receptors and hydroxylapatite and hydrophobic substrata. The last section also for bacterial lectins, and the lectins themselves. The use of deoxysugcontains three articles on the use of flow chamber devices and image ars to map the combining sites of bacterial lectins and the use of analysis, the measurement of bacterial adhesion by the subsequent synthetic peptides in the characterization of adhesins are also degrowth of adherent organisms in nutrient broth, and enzyme-linked scribed. Section three (six articles) provides general methods for visuimmunosorbent-based adhesion assays. alization of adhesion and its mathematic analysis. These articles As newcomers to the field of bacterial adhesion, my colleagues and discuss the use of electron and confocal microscopy, the mathematical I warmly welcome the appearance of this volume. This multiauthored treatment of binding data, various strategies for the quantitative compendium provides an introduction to many useful methods, and assay of bacterial adhesion to receptors, plant cells and tissues, mamit can be recommended to both novices and experts. malian cells and tissues, and an assay for adhesion of host cells to immobilized bacteria. Section four (four articles) provides methods for the molecular biological characterization of adhesins. These in-HOWARD GOLDFINE clude general strategies for employing molecular genetics, the use