Maximum volumetric production of β-galactosidase by Kluyveromyces fragilis in fed-batch culture with automatic control

✍ Scribed by Sonia Barberis; Rodolfo Segovia

• Publisher: Wiley (John Wiley & Sons)
• Year: 2002
• Tongue: English
• Weight: 126 KB
• Volume: 77
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.626

✦ Synopsis

Abstract

The production of β‐galactosidase by Kluyveromyces fragilis was studied in different culture systems, with dissolved oxygen concentration control and using defined media. An operating strategy of fed‐batch culture with automatic control of substrate addition regulated by dissolved oxygen concentration, consisting of the replacement of variable volumes of broth by fresh medium (once the fed‐batch culture has finished), was designed. The volumetric enzyme productivity (Q~p~, 13 600 UI dm^−3^ h^−1^) obtained was 38% higher than that reached in continuous culture of K fragilis with dissolved oxygen concentration control and far higher than that obtained by batch culture of K fragilis under the same aeration conditions.

© 2002 Society of Chemical Industry