Maturation-dependent reduced responsiveness of intracellular free Ca2+ ions to repeated stimulation by N-methyl-d-aspartate in cultured rat cortical neurons

Dual mechanisms of Ca2+ increases elicit

Dual mechanisms of Ca2+ increases elicited by N-methyl-D-aspartate in immature and mature cultured cortical neurons

✍ Noritaka Nakamichi; Hiroshi Ohno; Nobuyuki Kuramoto; Yukio Yoneda 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 395 KB

## Abstract Cortical primary cultures were loaded with the fluorescent indicator fluo‐3 for assessment of intracellular‐free Ca^2+^ ions with the aid of a confocal laser‐scanning microscope. The addition of __N__‐methyl‐D‐aspartic acid (NMDA) markedly increased the number of fluorescent cells in a