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Matrix design for matrix-assisted laser desorption ionization: Sensitive determination of PAH-DNA adducts

✍ Scribed by M. George; J.M.Y. Wellemans; R.L. Cerny; M.L. Gross; K. Li; E.L. Cavalieri

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
446 KB
Volume
5
Category
Article
ISSN
1044-0305

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✦ Synopsis

Two matrices, 4-phenyl-Ξ±-eyanocinnamic acid (PCC) and 4-benzyloxy-Ξ±-eyanocinnamic acid (BCC), were identified for the determination of polycyclic aromatic hydrocarbon (PAH) adducts of DNA bases by matrix-assisted laser desorption ionization. These matrices were designed based on the concept that the matrix and the analyte should have structural similarities. PCC is a good matrix for the desorption of not only PAH-modified DNA bases, but also PAHs themselves and their metabolites. Detections can be made at the femtomolar level.

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