Mating reaction inSaccharomyces cerevisiae. X Agglutinability-inactivating factor: A factor which destroys sexual agglutinability ofamating-type cells
✍ Scribed by Chikashi Shimoda; Yohkazu Matsushima; Naohiko Yanagishima
- Publisher
- Springer Netherlands
- Year
- 1976
- Tongue
- English
- Weight
- 544 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0003-6072
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✦ Synopsis
From cells of Saccharomyces cerevisiae a factor has been extracted that destroys the agglutinability of a mating-type cells specifically. It was found in the cell extracts of diploid and tetraploid strains as well as haploid strains of a and ~ mating types. It is heat-labile and the molecular weight is about 50000. It is adsorbed by neither a cells nor ~ cells. Its biological activity is dependent on the incubation temperature and the pH, and is completely inhibited by phenylmethylsulfonyl fluoride, a potent inhibitor of the serine proteases. All the results described in this paper indicate that this factor is a proteolytic enzyme.