Mathematical simulation of steady state isoelectric focusing of proteins using carrier ampholytes
β Scribed by Dr. Kazuo Shimao
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 487 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0173-0835
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## Steady-state concentration distribution of ampholytes in isoelectric focusing in a linear immobilized pH gradient The equation of balance between electrophoretic and diffusional mass flows in the steady state of isoelectric focusing is analyzed. To create the pH gradient, a model system compose
## Abstract A simple technique for polyacrylamide gel isoelectric focusing of Ξ±~1~βantitrypsin (PI) using thin, highly crossβlinked gels, restricted pHβrange carrier ampholytes and a separator was developed. This method enables easy typing of all known PI phenotypes including the four PI\*M subtype