Mathematical modelling of two-analyte sequential determinations by flow-injection sandwich techniques

✍ Scribed by J.L. Montesinos; J. Alonso; M. del Valle; J.L.F.C. Lima; M. Poch

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 903 KB
• Volume: 254
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(91)90024-y

✦ Synopsis

AbStlXt

Results obtained in the evaluation of a mathematical model for flow-injection sandwich systems, in which the sample is inserted between two different carrier solutions with the aid of an eight-way injection valve, are presented. The model considers the system as a tubular reactor with axially dispersed plug flow, where chemical kinetics have been included. Only a minimum of parameters are needed for the model: the dispersion coefficients, which are obtained from experimental correlations for the different hydrodynamic conditions, and the rate constants, which are determined by univariate optimization. The behaviour of the model, when applied to the case of one analyte and one reagent, was evaluated for the enzymatic determinations of glucose or glycerol. The case of two analytes determined with two reagents was also studied in the combined determination of glucose and glycerol. Experimental profiles were compared with the simulated peaks and showed good agreement.