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Mathematical Modeling the Kinetics of Cell Distribution in the Process of Ligand–Receptor Binding

✍ Scribed by IVAN V SUROVTSEV; IVAN A RAZUMOV; VYACHESLAV M NEKRASOV; ALEXANDER N SHVALOV; JUHANI T SOINI; VALERI P MALTSEV; ALEXANDER K PETROV; VALERI B LOKTEV; ANDREI V CHERNYSHEV

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
195 KB
Volume
206
Category
Article
ISSN
0022-5193

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✦ Synopsis

A statistical approach is presented to model the kinetics of cell distribution in the process of ligand}receptor binding on cell surfaces. The approach takes into account the variation of the amount of receptors on cells assuming the homogeneity of monovalent binding sites and ligand molecules. The analytical expressions for the kinetics of cell distribution have been derived in the reaction-limited approximation. In order to demonstrate the applicability of the mathematical model, the kinetics of binding the rabbit, anti-mouse IgG with Ig-receptors of the murine hybridoma cells has been measured. Anti-mouse IgG was labeled with #uorescein isothiocyanate (FITC). The kinetics of cell distribution on ligand}receptor complexes was observed during the reaction process by real-time measuring of the #uorescence and lightscattering traces of individual cells with the scanning #ow cytometer. The experimental data were "tted by the mathematical model in order to obtain the binding rate constant and the initial cell distribution on the amount of receptors.

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