Maternal effect of low temperature on handedness determination inC. elegans embryos

✍ Scribed by Wood, William B. ;Bergmann, Dominique ;Florance, Amy

• Book ID: 102660664
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 894 KB
• Volume: 19
• Category: Article
• ISSN: 0192-253X
• DOI: 10.1002/(sici)1520-6408(1996)19:3<222::aid-dvg5>3.0.co;2-b

✦ Synopsis

C. elegans embryos, larvae, and adults exhibit several left-right asymmetries with an invariant dextral handedness, which first becomes evident in the embryo at the 6-cell stage. Reversed (sinistral) handedness was not observed among >lO,OOO N2 adults reared at 16°C or 20°C under standard conditions. However, among the progeny of adults reproducing at 10"C, the frequency of animals with sinistral handedness was increased to -0.5%. Cold pulse experiments indicated that the critical period for this increase was in early oogenesis, several hours before the first appearance of left-right asymmetry in the embryo.

Hermaphrodites reared at 10°C and mated with males reared at 20°C produced sinistral outcross as well as sinistral self-progeny, indicating that the low temperature effect on oocytes was sufficient to cause reversals. Increased frequency of reversal was also observed among animals developed from embryos lacking the egg shell. Possible mechanisms for the control of embryonic handedness are discussed in the context of these results, including the hypothesis that handedness could be dictated by the chirality of a gametic component.