Mast cell involvement in normal human skin wound healing: expression of monocyte chemoattractant protein-1 is correlated with recruitment of mast cells which synthesize interleukin-4in vivo
✍ Scribed by Trautmann, Axel; Toksoy, Atiye; Engelhardt, Eva; Br�cker, Eva-B.; Gillitzer, Reinhard
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 340 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0022-3417
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✦ Synopsis
Mast cells (MCs) are known as key cells of immediate type hypersensitivity reactions. It has recently been shown that MCs regulate ®broblast proliferation by heterotypic cell±cell contact and secretion of interleukin-4 (IL-4) in vitro. It was therefore hypothesized that MCs may contribute to wound repair in vivo. Using immunohistology and in situ hybridization, the time course of mast cell recruitment and the expression of MC-attractant chemokines were analysed in a human skin wound-healing model, and the production of IL-4 by MCs in vivo was investigated. The data obtained indicate that the ®ve-fold increase of the tryptase+ MCs at the ®brotic border of the wound within the ®rst 10 days is the result of increased recruitment/survival of MCs or MC precursors, but not of increased local proliferation. Recruitment of MCs is paralleled by the expression of monocyte chemoattractant protein-1 (MCP-1), but not by other chemokines such as RANTES (regulated on activation, normal T cell expressed and secreted) and/or MIP (macrophage in¯ammatory protein)-1a/b. Notably, 60±70% of MCs exhibited strong and selective IL-4 immunoreactivity, whereas other resident and passenger cells were rather quiescent. The data suggest that MC contribute signi®cantly to the cytokine network of wound repair via MC-derived IL-4 and stimulation of ®broblast proliferation.