Massive cytoplasmic transfers between early blastomeres ofRana pipiens eggs
✍ Scribed by Ansevin, Krystyna D. ;Williams, Barbara J.
- Book ID
- 102335225
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 329 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
A technique for injecting large amounts of cytoplasm into early blastomeres of frog eggs is reported. It has been possible to replace part of the cytoplasm of animal blastomeres at the 8‐cell stage with injected cytoplasm, some of which was also included in the upper region of the corresponding cell in the vegetal hemisphere. When the injected cytoplasm matched the stage and type of the cytoplasm at the site of injection, the majority of the recipient embryos developed into normal larvae. If it matched the stage, but not the type, the majority of the eggs developed abnormally. The experiments also indicated a relationship between the stage of injected cytoplasm (4‐64+ cells) and the frequency and severity of abnormalities.